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abclonal first strand synthesis buffer  (ABclonal Biotechnology)


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    ABclonal Biotechnology abclonal first strand synthesis buffer
    Abclonal First Strand Synthesis Buffer, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/first-strand+buffer/5X+First-Strand+Buffer/pm42514934-89-45-45
    Average 95 stars, based on 10 article reviews
    abclonal first strand synthesis buffer - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Sequencing:

    Article Title: ApiRegenin, an Animal-Derived Platelet-Rich Plasma Extract, Accelerates Wound Healing of Chronic Diabetic Ulcer in Mice.
    Article Snippet: Total RNA was isolated from skin tissues with TRIzol® Reagent following the manufacturer’s protocol, RNA purity was verified by the A260/A280 absorbance ratio using a Nanodrop ND 2000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA) and RNA integrity number (RIN) was assessed with an Agilent Bioanalyzer 4150 system (Agilent Technologies, Santa Clara, CA, USA), with only qualified RNA samples (RNA Integrity Number, RIN ≥ 7.0) used for library construction; for each group, three independent biological replicates (n = 3) were processed. .. Paired-end sequencing libraries were prepared with the VAHTS Universal V10 RNA-seq Library Prep Kit for Illumina (Vazyme, Nanjing, China) per the manufacturer’s instructions, involving oligo (dT) magnetic bead enrichment of mRNA from 1 μg total RNA, mRNA fragmentation with divalent cations at high temperatures in ABclonal first-strand synthesis buffer, synthesis of first-strand cDNA with random hexamer primers and RNase H minus reverse transcriptase, generation of secondstrand cDNA with DNA polymerase I, RNase H, reaction buffer and dNTPs, ligation of double-stranded cDNA fragments with sequencing adapters, PCR amplification for library enrichment, purification of amplified PCR products and validation of library quality via the Agilent Bioanalyzer 4150 system. .. High-throughput sequencing was executed on an Illumina NovaSeq/MGISEQ T7 platform, yielding an average read depth of ≥45 M raw reads per sample.

    RNA Sequencing:

    Article Title: ApiRegenin, an Animal-Derived Platelet-Rich Plasma Extract, Accelerates Wound Healing of Chronic Diabetic Ulcer in Mice.
    Article Snippet: Total RNA was isolated from skin tissues with TRIzol® Reagent following the manufacturer’s protocol, RNA purity was verified by the A260/A280 absorbance ratio using a Nanodrop ND 2000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA) and RNA integrity number (RIN) was assessed with an Agilent Bioanalyzer 4150 system (Agilent Technologies, Santa Clara, CA, USA), with only qualified RNA samples (RNA Integrity Number, RIN ≥ 7.0) used for library construction; for each group, three independent biological replicates (n = 3) were processed. .. Paired-end sequencing libraries were prepared with the VAHTS Universal V10 RNA-seq Library Prep Kit for Illumina (Vazyme, Nanjing, China) per the manufacturer’s instructions, involving oligo (dT) magnetic bead enrichment of mRNA from 1 μg total RNA, mRNA fragmentation with divalent cations at high temperatures in ABclonal first-strand synthesis buffer, synthesis of first-strand cDNA with random hexamer primers and RNase H minus reverse transcriptase, generation of secondstrand cDNA with DNA polymerase I, RNase H, reaction buffer and dNTPs, ligation of double-stranded cDNA fragments with sequencing adapters, PCR amplification for library enrichment, purification of amplified PCR products and validation of library quality via the Agilent Bioanalyzer 4150 system. .. High-throughput sequencing was executed on an Illumina NovaSeq/MGISEQ T7 platform, yielding an average read depth of ≥45 M raw reads per sample.

    Random Hexamer:

    Article Title: ApiRegenin, an Animal-Derived Platelet-Rich Plasma Extract, Accelerates Wound Healing of Chronic Diabetic Ulcer in Mice.
    Article Snippet: Total RNA was isolated from skin tissues with TRIzol® Reagent following the manufacturer’s protocol, RNA purity was verified by the A260/A280 absorbance ratio using a Nanodrop ND 2000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA) and RNA integrity number (RIN) was assessed with an Agilent Bioanalyzer 4150 system (Agilent Technologies, Santa Clara, CA, USA), with only qualified RNA samples (RNA Integrity Number, RIN ≥ 7.0) used for library construction; for each group, three independent biological replicates (n = 3) were processed. .. Paired-end sequencing libraries were prepared with the VAHTS Universal V10 RNA-seq Library Prep Kit for Illumina (Vazyme, Nanjing, China) per the manufacturer’s instructions, involving oligo (dT) magnetic bead enrichment of mRNA from 1 μg total RNA, mRNA fragmentation with divalent cations at high temperatures in ABclonal first-strand synthesis buffer, synthesis of first-strand cDNA with random hexamer primers and RNase H minus reverse transcriptase, generation of secondstrand cDNA with DNA polymerase I, RNase H, reaction buffer and dNTPs, ligation of double-stranded cDNA fragments with sequencing adapters, PCR amplification for library enrichment, purification of amplified PCR products and validation of library quality via the Agilent Bioanalyzer 4150 system. .. High-throughput sequencing was executed on an Illumina NovaSeq/MGISEQ T7 platform, yielding an average read depth of ≥45 M raw reads per sample.

    Reverse Transcription:

    Article Title: ApiRegenin, an Animal-Derived Platelet-Rich Plasma Extract, Accelerates Wound Healing of Chronic Diabetic Ulcer in Mice.
    Article Snippet: Total RNA was isolated from skin tissues with TRIzol® Reagent following the manufacturer’s protocol, RNA purity was verified by the A260/A280 absorbance ratio using a Nanodrop ND 2000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA) and RNA integrity number (RIN) was assessed with an Agilent Bioanalyzer 4150 system (Agilent Technologies, Santa Clara, CA, USA), with only qualified RNA samples (RNA Integrity Number, RIN ≥ 7.0) used for library construction; for each group, three independent biological replicates (n = 3) were processed. .. Paired-end sequencing libraries were prepared with the VAHTS Universal V10 RNA-seq Library Prep Kit for Illumina (Vazyme, Nanjing, China) per the manufacturer’s instructions, involving oligo (dT) magnetic bead enrichment of mRNA from 1 μg total RNA, mRNA fragmentation with divalent cations at high temperatures in ABclonal first-strand synthesis buffer, synthesis of first-strand cDNA with random hexamer primers and RNase H minus reverse transcriptase, generation of secondstrand cDNA with DNA polymerase I, RNase H, reaction buffer and dNTPs, ligation of double-stranded cDNA fragments with sequencing adapters, PCR amplification for library enrichment, purification of amplified PCR products and validation of library quality via the Agilent Bioanalyzer 4150 system. .. High-throughput sequencing was executed on an Illumina NovaSeq/MGISEQ T7 platform, yielding an average read depth of ≥45 M raw reads per sample.

    Ligation:

    Article Title: ApiRegenin, an Animal-Derived Platelet-Rich Plasma Extract, Accelerates Wound Healing of Chronic Diabetic Ulcer in Mice.
    Article Snippet: Total RNA was isolated from skin tissues with TRIzol® Reagent following the manufacturer’s protocol, RNA purity was verified by the A260/A280 absorbance ratio using a Nanodrop ND 2000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA) and RNA integrity number (RIN) was assessed with an Agilent Bioanalyzer 4150 system (Agilent Technologies, Santa Clara, CA, USA), with only qualified RNA samples (RNA Integrity Number, RIN ≥ 7.0) used for library construction; for each group, three independent biological replicates (n = 3) were processed. .. Paired-end sequencing libraries were prepared with the VAHTS Universal V10 RNA-seq Library Prep Kit for Illumina (Vazyme, Nanjing, China) per the manufacturer’s instructions, involving oligo (dT) magnetic bead enrichment of mRNA from 1 μg total RNA, mRNA fragmentation with divalent cations at high temperatures in ABclonal first-strand synthesis buffer, synthesis of first-strand cDNA with random hexamer primers and RNase H minus reverse transcriptase, generation of secondstrand cDNA with DNA polymerase I, RNase H, reaction buffer and dNTPs, ligation of double-stranded cDNA fragments with sequencing adapters, PCR amplification for library enrichment, purification of amplified PCR products and validation of library quality via the Agilent Bioanalyzer 4150 system. .. High-throughput sequencing was executed on an Illumina NovaSeq/MGISEQ T7 platform, yielding an average read depth of ≥45 M raw reads per sample.

    Polymerase Chain Reaction:

    Article Title: ApiRegenin, an Animal-Derived Platelet-Rich Plasma Extract, Accelerates Wound Healing of Chronic Diabetic Ulcer in Mice.
    Article Snippet: Total RNA was isolated from skin tissues with TRIzol® Reagent following the manufacturer’s protocol, RNA purity was verified by the A260/A280 absorbance ratio using a Nanodrop ND 2000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA) and RNA integrity number (RIN) was assessed with an Agilent Bioanalyzer 4150 system (Agilent Technologies, Santa Clara, CA, USA), with only qualified RNA samples (RNA Integrity Number, RIN ≥ 7.0) used for library construction; for each group, three independent biological replicates (n = 3) were processed. .. Paired-end sequencing libraries were prepared with the VAHTS Universal V10 RNA-seq Library Prep Kit for Illumina (Vazyme, Nanjing, China) per the manufacturer’s instructions, involving oligo (dT) magnetic bead enrichment of mRNA from 1 μg total RNA, mRNA fragmentation with divalent cations at high temperatures in ABclonal first-strand synthesis buffer, synthesis of first-strand cDNA with random hexamer primers and RNase H minus reverse transcriptase, generation of secondstrand cDNA with DNA polymerase I, RNase H, reaction buffer and dNTPs, ligation of double-stranded cDNA fragments with sequencing adapters, PCR amplification for library enrichment, purification of amplified PCR products and validation of library quality via the Agilent Bioanalyzer 4150 system. .. High-throughput sequencing was executed on an Illumina NovaSeq/MGISEQ T7 platform, yielding an average read depth of ≥45 M raw reads per sample.

    Amplification:

    Article Title: ApiRegenin, an Animal-Derived Platelet-Rich Plasma Extract, Accelerates Wound Healing of Chronic Diabetic Ulcer in Mice.
    Article Snippet: Total RNA was isolated from skin tissues with TRIzol® Reagent following the manufacturer’s protocol, RNA purity was verified by the A260/A280 absorbance ratio using a Nanodrop ND 2000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA) and RNA integrity number (RIN) was assessed with an Agilent Bioanalyzer 4150 system (Agilent Technologies, Santa Clara, CA, USA), with only qualified RNA samples (RNA Integrity Number, RIN ≥ 7.0) used for library construction; for each group, three independent biological replicates (n = 3) were processed. .. Paired-end sequencing libraries were prepared with the VAHTS Universal V10 RNA-seq Library Prep Kit for Illumina (Vazyme, Nanjing, China) per the manufacturer’s instructions, involving oligo (dT) magnetic bead enrichment of mRNA from 1 μg total RNA, mRNA fragmentation with divalent cations at high temperatures in ABclonal first-strand synthesis buffer, synthesis of first-strand cDNA with random hexamer primers and RNase H minus reverse transcriptase, generation of secondstrand cDNA with DNA polymerase I, RNase H, reaction buffer and dNTPs, ligation of double-stranded cDNA fragments with sequencing adapters, PCR amplification for library enrichment, purification of amplified PCR products and validation of library quality via the Agilent Bioanalyzer 4150 system. .. High-throughput sequencing was executed on an Illumina NovaSeq/MGISEQ T7 platform, yielding an average read depth of ≥45 M raw reads per sample.

    Purification:

    Article Title: ApiRegenin, an Animal-Derived Platelet-Rich Plasma Extract, Accelerates Wound Healing of Chronic Diabetic Ulcer in Mice.
    Article Snippet: Total RNA was isolated from skin tissues with TRIzol® Reagent following the manufacturer’s protocol, RNA purity was verified by the A260/A280 absorbance ratio using a Nanodrop ND 2000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA) and RNA integrity number (RIN) was assessed with an Agilent Bioanalyzer 4150 system (Agilent Technologies, Santa Clara, CA, USA), with only qualified RNA samples (RNA Integrity Number, RIN ≥ 7.0) used for library construction; for each group, three independent biological replicates (n = 3) were processed. .. Paired-end sequencing libraries were prepared with the VAHTS Universal V10 RNA-seq Library Prep Kit for Illumina (Vazyme, Nanjing, China) per the manufacturer’s instructions, involving oligo (dT) magnetic bead enrichment of mRNA from 1 μg total RNA, mRNA fragmentation with divalent cations at high temperatures in ABclonal first-strand synthesis buffer, synthesis of first-strand cDNA with random hexamer primers and RNase H minus reverse transcriptase, generation of secondstrand cDNA with DNA polymerase I, RNase H, reaction buffer and dNTPs, ligation of double-stranded cDNA fragments with sequencing adapters, PCR amplification for library enrichment, purification of amplified PCR products and validation of library quality via the Agilent Bioanalyzer 4150 system. .. High-throughput sequencing was executed on an Illumina NovaSeq/MGISEQ T7 platform, yielding an average read depth of ≥45 M raw reads per sample.

    Article Title: Targeting <scp>YAP1</scp> ‐Mediated Tubular Cell Senescence to Attenuate Maladaptive Repair and Fibrotic Transition After Acute Kidney Injury
    Article Snippet: Library preparation and Sequencing: Paired- end libraries were prepared using an ABclonal mRNA- seq Lib Prep Kit (ABclonal, China) following the manufacturer's instructions. .. The mRNA was purified from 1 μg total RNA using oligo (dT) magnetic beads followed by fragmentation carried out using divalent cations at elevated temperatures in ABclonal First Strand Synthesis Reaction Buffer. ..

    Article Title: Classical swine fever virus hijacks triglyceride metabolism via the NS5B-PLIN5 axis to facilitate viral replication.
    Article Snippet: Classical swine fever virus (CSFV) represents a critical pathogen that causes substantial economic losses to the swine industry and heavily relies on host lipid metabolic reprogramming during infection.. However, the detailed regulatory mechanisms governing CSFV-induced lipid metabolic remodeling remain poorly understanding.. In this study, we systematically investigated how CSFV reprograms lipid metabolism to facilitate viral replication.

    Biomarker Discovery:

    Article Title: ApiRegenin, an Animal-Derived Platelet-Rich Plasma Extract, Accelerates Wound Healing of Chronic Diabetic Ulcer in Mice.
    Article Snippet: Total RNA was isolated from skin tissues with TRIzol® Reagent following the manufacturer’s protocol, RNA purity was verified by the A260/A280 absorbance ratio using a Nanodrop ND 2000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA) and RNA integrity number (RIN) was assessed with an Agilent Bioanalyzer 4150 system (Agilent Technologies, Santa Clara, CA, USA), with only qualified RNA samples (RNA Integrity Number, RIN ≥ 7.0) used for library construction; for each group, three independent biological replicates (n = 3) were processed. .. Paired-end sequencing libraries were prepared with the VAHTS Universal V10 RNA-seq Library Prep Kit for Illumina (Vazyme, Nanjing, China) per the manufacturer’s instructions, involving oligo (dT) magnetic bead enrichment of mRNA from 1 μg total RNA, mRNA fragmentation with divalent cations at high temperatures in ABclonal first-strand synthesis buffer, synthesis of first-strand cDNA with random hexamer primers and RNase H minus reverse transcriptase, generation of secondstrand cDNA with DNA polymerase I, RNase H, reaction buffer and dNTPs, ligation of double-stranded cDNA fragments with sequencing adapters, PCR amplification for library enrichment, purification of amplified PCR products and validation of library quality via the Agilent Bioanalyzer 4150 system. .. High-throughput sequencing was executed on an Illumina NovaSeq/MGISEQ T7 platform, yielding an average read depth of ≥45 M raw reads per sample.

    Lysis:

    Article Title: SETDB1 enhances starvation-induced lipophagy by inhibiting m 6 A-mediated mRNA decay via DDX5 methylation.
    Article Snippet: Lipophagy, a selective form of macroautophagy/autophagy, degrades lipid droplets (LDs) to provide energy and is implicated in metabolic disorders.. The molecular mechanism underlying lipophagy induction remains incompletely understood.. This study explored the role of SETDB1 in starvationinduced autophagy and lipophagy.

    Western Blot:

    Article Title: SETDB1 enhances starvation-induced lipophagy by inhibiting m 6 A-mediated mRNA decay via DDX5 methylation.
    Article Snippet: Lipophagy, a selective form of macroautophagy/autophagy, degrades lipid droplets (LDs) to provide energy and is implicated in metabolic disorders.. The molecular mechanism underlying lipophagy induction remains incompletely understood.. This study explored the role of SETDB1 in starvationinduced autophagy and lipophagy.

    Magnetic Beads:

    Article Title: SETDB1 enhances starvation-induced lipophagy by inhibiting m 6 A-mediated mRNA decay via DDX5 methylation.
    Article Snippet: Lipophagy, a selective form of macroautophagy/autophagy, degrades lipid droplets (LDs) to provide energy and is implicated in metabolic disorders.. The molecular mechanism underlying lipophagy induction remains incompletely understood.. This study explored the role of SETDB1 in starvationinduced autophagy and lipophagy.

    Article Title: Zha-Chong Shi-San-Wei Pill inhibits ferroptosis in cerebral ischemia via bioinformatics and experimental confirmation methods.
    Article Snippet: Ethnopharmacological relevance: Ischemic stroke (IS), accounting for approximately 85% of all stroke cases, is a cerebrovascular catastrophe that inflicts cerebral parenchymal damage through multifactorial mechanisms, resulting in millions of deaths and long-term neurological disabilities worldwide.. Zha-Chong Shi-San-Wei Pill (ZCSSW), a compound formulation derived from a synergistic blend of Mongolian medicinal herbs, has demonstrated significant therapeutic efficacy in managing IS.. Aim of the study: This study aims to investigate whether ZCSSW inhibits ferroptosis in cerebral ischemia (CI) via the hypoxia-inducible factor-1α (HIF-1α)/heme oxygenase-1 (HO-1) pathway.

    Article Title: Integrating metabolomics and transcriptomics to investigate the effects of changes in renal adenosine 5′-monophosphate and arginine metabolism on the mTOR pathway under chronic hypoxia
    Article Snippet: Paired-end (PE) libraries were prepared according to the manufacturer’s instructions of the ABclonal mRNA Seq Lib Prep Kit (ABclonal, China). .. The mRNA was extracted from 1 μg of total RNA using oligo (dT) magnetic beads and then fragmented in ABclonal First Strand Synthesis Reaction Buffer. ..

    Article Title: Targeting <scp>YAP1</scp> ‐Mediated Tubular Cell Senescence to Attenuate Maladaptive Repair and Fibrotic Transition After Acute Kidney Injury
    Article Snippet: Library preparation and Sequencing: Paired- end libraries were prepared using an ABclonal mRNA- seq Lib Prep Kit (ABclonal, China) following the manufacturer's instructions. .. The mRNA was purified from 1 μg total RNA using oligo (dT) magnetic beads followed by fragmentation carried out using divalent cations at elevated temperatures in ABclonal First Strand Synthesis Reaction Buffer. ..

    Article Title: Classical swine fever virus hijacks triglyceride metabolism via the NS5B-PLIN5 axis to facilitate viral replication.
    Article Snippet: Classical swine fever virus (CSFV) represents a critical pathogen that causes substantial economic losses to the swine industry and heavily relies on host lipid metabolic reprogramming during infection.. However, the detailed regulatory mechanisms governing CSFV-induced lipid metabolic remodeling remain poorly understanding.. In this study, we systematically investigated how CSFV reprograms lipid metabolism to facilitate viral replication.

    Expressing:

    Article Title: SETDB1 enhances starvation-induced lipophagy by inhibiting m 6 A-mediated mRNA decay via DDX5 methylation.
    Article Snippet: Lipophagy, a selective form of macroautophagy/autophagy, degrades lipid droplets (LDs) to provide energy and is implicated in metabolic disorders.. The molecular mechanism underlying lipophagy induction remains incompletely understood.. This study explored the role of SETDB1 in starvationinduced autophagy and lipophagy.

    Construct:

    Article Title: SETDB1 enhances starvation-induced lipophagy by inhibiting m 6 A-mediated mRNA decay via DDX5 methylation.
    Article Snippet: Lipophagy, a selective form of macroautophagy/autophagy, degrades lipid droplets (LDs) to provide energy and is implicated in metabolic disorders.. The molecular mechanism underlying lipophagy induction remains incompletely understood.. This study explored the role of SETDB1 in starvationinduced autophagy and lipophagy.

    Generated:

    Article Title: SETDB1 enhances starvation-induced lipophagy by inhibiting m 6 A-mediated mRNA decay via DDX5 methylation.
    Article Snippet: Lipophagy, a selective form of macroautophagy/autophagy, degrades lipid droplets (LDs) to provide energy and is implicated in metabolic disorders.. The molecular mechanism underlying lipophagy induction remains incompletely understood.. This study explored the role of SETDB1 in starvationinduced autophagy and lipophagy.

    shRNA:

    Article Title: SETDB1 enhances starvation-induced lipophagy by inhibiting m 6 A-mediated mRNA decay via DDX5 methylation.
    Article Snippet: Lipophagy, a selective form of macroautophagy/autophagy, degrades lipid droplets (LDs) to provide energy and is implicated in metabolic disorders.. The molecular mechanism underlying lipophagy induction remains incompletely understood.. This study explored the role of SETDB1 in starvationinduced autophagy and lipophagy.

    RNA Immunoprecipitation:

    Article Title: SETDB1 enhances starvation-induced lipophagy by inhibiting m 6 A-mediated mRNA decay via DDX5 methylation.
    Article Snippet: Lipophagy, a selective form of macroautophagy/autophagy, degrades lipid droplets (LDs) to provide energy and is implicated in metabolic disorders.. The molecular mechanism underlying lipophagy induction remains incompletely understood.. This study explored the role of SETDB1 in starvationinduced autophagy and lipophagy.

    Real-time Polymerase Chain Reaction:

    Article Title: SETDB1 enhances starvation-induced lipophagy by inhibiting m 6 A-mediated mRNA decay via DDX5 methylation.
    Article Snippet: Lipophagy, a selective form of macroautophagy/autophagy, degrades lipid droplets (LDs) to provide energy and is implicated in metabolic disorders.. The molecular mechanism underlying lipophagy induction remains incompletely understood.. This study explored the role of SETDB1 in starvationinduced autophagy and lipophagy.

    Isolation:

    Article Title: SETDB1 enhances starvation-induced lipophagy by inhibiting m 6 A-mediated mRNA decay via DDX5 methylation.
    Article Snippet: Lipophagy, a selective form of macroautophagy/autophagy, degrades lipid droplets (LDs) to provide energy and is implicated in metabolic disorders.. The molecular mechanism underlying lipophagy induction remains incompletely understood.. This study explored the role of SETDB1 in starvationinduced autophagy and lipophagy.

    Article Title: Zha-Chong Shi-San-Wei Pill inhibits ferroptosis in cerebral ischemia via bioinformatics and experimental confirmation methods.
    Article Snippet: Ethnopharmacological relevance: Ischemic stroke (IS), accounting for approximately 85% of all stroke cases, is a cerebrovascular catastrophe that inflicts cerebral parenchymal damage through multifactorial mechanisms, resulting in millions of deaths and long-term neurological disabilities worldwide.. Zha-Chong Shi-San-Wei Pill (ZCSSW), a compound formulation derived from a synergistic blend of Mongolian medicinal herbs, has demonstrated significant therapeutic efficacy in managing IS.. Aim of the study: This study aims to investigate whether ZCSSW inhibits ferroptosis in cerebral ischemia (CI) via the hypoxia-inducible factor-1α (HIF-1α)/heme oxygenase-1 (HO-1) pathway.



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    Image Search Results


    Journal: STAR Protocols

    Article Title: Protocol to isolate broadly neutralizing monoclonal antibodies against SARS-CoV-2 from human B cells

    doi: 10.1016/j.xpro.2025.104275

    Figure Lengend Snippet: RT mix-II

    Article Snippet: 5× First-Strand Buffer (part of the SuperScript III Reverse Transcriptase) , Thermo Fisher Scientific , Cat#18080044.

    Techniques: